Xylanase deconstructs cell structural material by breaking down hemicellulose, a major component of a plant cell wall. Cell walls prevent dehydration and maintain physical integrity of the cell. In food we want to break down these walls to get at the nutr Breaks down fiber found primarily in grains in a pH of 3-7.
Xylanase converts hemicellulose to nutritive sugar that the body can digest, xylanase produces compounds that may be a nutritive source for the microflora.
Technicals: Xylanase enzyme (Endo-1,4-Beta-xylanase, or XYNII, EC 184.108.40.206) from Trichoderma sp has a pI of 9.0 and is produced by fermentation. Xylanase consists of 190 amino acids and has a molecular weight of 21 kD. Xylanases belong to the glucanase enzyme family, characterized by their ability to break down various xylans to produce short-chain xylo-oligosaccharides. Xylanase crystallizes in ammonium sulfate and sodium/potassium phosphate across pH 3.5 to 9.0. Xylanase can be crystallized with other salts, polymers, and organic solvents. Xylanase solubility increases with increasing temperature in moderate concentrations of ammonium sulfate. Xylanase solubility in phosphate pH 9 decreases in the temperature range of 0 to 10 degrees Celsius but remains constant in the range of 10 through 37 degrees Celsius. Xylanase has been extracted from fungi and bacteria.
Sources: Aspergillus niger
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